I International Symposium on Vegetative Propagation and In Vitro Culture of Tropical Plants

The great progress produced in the last century by the genetic improvement of plants would have been of little significance without the contemporary amelioration of the vegetative propagation technology. The use of portions of shoots to reproduce plants was already known in the early horticulture of Egypt, Babylon and China, while evidences of the “budding and grafting art” are frequent in writings and mosaics from the Roman Empire. With the important advancements in methods and equipments made in the twentieth century, coupled to a better understanding of physiological and morphological events driving the processes of adventitious root formation, graft healing and in vitro shoot proliferation, a range of effective techniques are available today for the in vivo and in vitro clonal reproduction of plants. Moreover, increasingly sophisticated methods of tissue culture paved the way to the development of useful approaches for the production and conservation of high-quality pathogen-free plants. Notwithstanding, many unsolved issues are still relevant, such as the “recalcitrancy” of many species to be clonally reproduced. The Symposium, the first of the series, aims to bring together scientists and students, as well as managers and technicians from commercial nurseries and micropropagation laboratories, around the problematics and recent acquisitions in the field of vegetative propagation and in vitro culture of tropical and sub-tropical plant species.


  1. Vegetative Propagation (cutting, grafting) of Tropical Fruit Plants
  2. Vegetative Propagation (cutting, grafting) of Tropical Forest, Ornamental and Medicinal Plants
  3. Micropropagation of Tropical Plants: Innovation and Large Scale Production
  4. Somatic Embryogenesis in Tropical Plants
  5. In Vitro Conservation of Tropical Plants


Professor Maurizio Lambardi

is a Senior scientist and project leader of the National Research Council (CNR) of Italy, at the Trees and Timber Institute (IVALSA) of Florence, and has the National Scientific Qualification of Full Professor in arboriculture and forest systems. He is the Chair of the ISHS Commission ‘Molecular Biology and In Vitro Culture’ and of the Italian Working Group on ‘Micropropagation and In Vitro Technologies’, and he is past-Chair of the Society for Low Temperature Biology (SLTB). Read more.

Image of Professor Renato PaivaProfessor Renato Paiva

has a Master’s in Agronomy at the Mississippi State University (USA) and PhD also in Agronomy at the University of Illinois (USA).

Nowadays, he is a Full Professor of Plant Physiology at the Federal University of Lavras (UFLA). He teaches courses, advise undergraduate and graduate students, and perform research related to the micropropagation and in vitro conservation of native Brazilian fruit species. Read more.

Scientific Committee

  • Adel A. Abul-Soad, Agriculture Research Centre, Giza, Egypt
  • Jorge Canhoto, University of Coimbra, Portugal
  • Roderick Drew, Griffith University, Brisbane, Australia
  • Sharon Hamill, Agri-Science Queensland, Nambour, Australia
  • Edwin Herman, Agricell Report, USA
  • Maria Antonietta Germanà, University of Palermo, Italy
  • Miguel Pedro Guerra, Federal University of Santa Catarina, Florianópolis, Brazil
  • Ivan Iliev, University of Forestry, Sofia, Bulgaria
  • Sisir Mitra, ISHS Section Chair, India
  • John Preece, USDA-ARS NCGR, Davis, USA
  • Anabela Romano, University of Algarve, Faro, Portugal
  • Takuya Tetsumura, University of Miyazaki, Japan

No. Title Author
1 Production of Phytoplasma-free Plants in Sugarcane (Saccharum spp.) using Temporary Immersion Bioreactor Karsedis Distabanjong
2 Recent Development in the Propagation of Tropical and Subtropical Fruit Crops by cutting Prof. Dr. Sisir Kumar Mitra
3 Micro-tuber Induction of Elephant Yam (Amorphophallus oncophyllus) Dr. Chockpisit Thepsithar
4 The use of Sucrose and Indole-3-Butyric Acid for Increasing Quantity of Root and Acclimatization of Ant Plant (Myrmecodia pendans) Dr. Innaka Ageng Rineksane
5 Oryzalin induction and identification of mutant genes in Anthurium using differential display RT-PCR (DDRT-PCR) Assist. Prof. Potjamarn Suraninpong
6 In vitro Propagation of Subtropical Yam, Dioscorea alata L., and their Culture under Temperate Climate Dr. Shizuka Ohki
7 In vitro rejuvenation of physiologically mature avocado material through meristem culture Ms. Jayeni Hiti Bandaralage
8 An effective indexing method for banana tissue culture provides long-term freedom from bacterial contamination Sharon Hamill
9 Fusarium oxysporum f.sp. cubense rapidly infects and kills both susceptible and resistant banana cultivars in tissue culture Sharon Hamill
10 Cryopreservation of Avocado (Persea Americana Mill.) for Somatic Embryos and Meristems using Vitrification Techniques Mr. Chris O’Brien
11 Temperature, photoperiod and colored shade nets on in vitro development of Physalis peruviana L Prof. Dr. Patrícia Duarte de Oliveira Paiva
12 In Vitro Culture of Tropical Tree Species Prof. Dr. Roderick A. Drew
13 In vitro propagation of Clivia miniata (Lindl.) Regel var. miniata, an indigenous South African species from Seedling derived explants Viloshanie Reddy
14 Strategies for maintaining and increasing throughput of in vitro cultures of sugarcane Dr. Sandra Snyman
1 Callogenesis induction in nucellar explant of Theobroma grandiflorum Regina Caetano Quisen
2 Multiple shoot induction of pomelo [Citrus maxima (Burm.) Merr.] `Thongdee´ Assist. Prof. Kullanart Obsuwan
3 Effects of known and unknown molecular mass chitosan products on plantlets development of Dendrobium and Cattleya hybrids Assist. Prof. Budsaraporn Ngampanya
4 Effects of Porcine Oviductal Fluid on growth of Dendrobium mirbelianum in vitro Assist. Prof. Chockpisit Thepsithar
5 A Novel Approach of Producing Pre-basic and Basic Seed Yam through Tissue Culture Micropropagation Mr. David Appiah-Kubi
6 Effects of p-chlorophenoxyisobutyric acid and 2,3,5-triiodobenzoic acid on in vitro micropropagation of Aglaonema ‘Lady Valentine’ Ping-Lung Huang
7 Towards a cryopreserved germplasm collection of apple ‘ results of dormant bud cryopreservation in the mild maritime winter climate of Hawkes Bay, New Zealand Dr. Ranjith Pathirana


International Symposia on Tropical and Temperate Horticulture